| Autor: |
Page WJ; Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada T6G 2E9. bill.page@ualberta.ca, Kwon E, Cornish AS, Tindale AE |
| Jazyk: |
angličtina |
| Zdroj: |
FEMS microbiology letters [FEMS Microbiol Lett] 2003 Nov 21; Vol. 228 (2), pp. 211-6. |
| DOI: |
10.1016/S0378-1097(03)00753-5 |
| Abstrakt: |
The csbX gene of Azotobacter vinelandii was regulated in an iron-repressible manner from a divergent promoter upstream of the catecholate siderophore biosynthesis (csb) operon and was predicted to encode an efflux pump of the major facilitator superfamily. Other proteins that were most similar to CsbX were encoded by genes found in the catecholate siderophore biosynthesis operons of Aeromonas hydrophila and Stigmatella aurantiaca. Inactivation of csbX resulted in 57-100% decrease in the amount of catecholates released when compared to the wild-type in iron-limited medium. CsbX was most important for the export of the high affinity chelator protochelin with the majority of the catecholates released by csbX mutants being the protochelin intermediates azotochelin and aminochelin. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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