| Autor: |
Gottumukkala RV; Division of Clinical Sciences (North), University of Sheffield, Sheffield, UK., Waterman EA, Herd LM, Gawkrodger DJ, Watson PF, Weetman AP, Kemp EH |
| Jazyk: |
angličtina |
| Zdroj: |
The Journal of investigative dermatology [J Invest Dermatol] 2003 Oct; Vol. 121 (4), pp. 765-70. |
| DOI: |
10.1046/j.1523-1747.2003.12494.x |
| Abstrakt: |
Previously, we reported the identification of autoantibodies against the melanin-concentrating hormone receptor 1 in patients with vitiligo. In this study, the B cell epitopes on melanin-concentrating hormone receptor 1 that are recognized by these autoantibodies have been identified. Deletion derivatives of melanin-concentrating hormone receptor 1 complementary DNA were constructed and then translated in vitro with the concomitant incorporation of [35S]-methionine into the protein products. The [35S]-labeled melanin-concentrating hormone receptor 1 derivatives were subsequently used in radio-binding assays to investigate the reactivity of sera from nine vitiligo patients that were known to contain antibodies to the receptor. Analysis of the results obtained in the radio-binding assays suggested the existence of multiple antibody binding sites on melanin-concentrating hormone receptor 1, including regions between amino acids 1 to 138 and 139 to 298. Several patients exhibited autoantibodies to more than one melanin-concentrating hormone receptor 1 epitope indicating a heterogeneous humoral response to the receptor. Computer prediction of the potential B cell epitopes on melanin-concentrating hormone receptor 1 revealed that the epitope domains identified overlapped, at least in part, with regions predicted to be highly antigenic. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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