| Autor: |
Yu YQ; Life Sciences Research and Development, Waters Corporation, 34 Maple Street, Milford, Massachusetts 01757, USA., Gilar M, Lee PJ, Bouvier ES, Gebler JC |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical chemistry [Anal Chem] 2003 Nov 01; Vol. 75 (21), pp. 6023-8. |
| DOI: |
10.1021/ac0346196 |
| Abstrakt: |
Improved in-solution tryptic digestion of proteins in terms of speed and peptide coverage was achieved with the aid of a novel acid-labile anionic surfactant (ALS). Unlike SDS, ALS solubilizes proteins without inhibiting trypsin or other common endopeptidases activity. Trypsin activity was evaluated in the presence of various denaturants; little or no decrease in proteolytic activity was observed in 0.1-1% ALS solutions (w/v). Sample preparation prior to mass spectrometry and liquid chromatography analysis consists of sample acidification. ALS degrades rapidly at low-pH conditions, which eliminates surfactant-caused interference with analysis. Described methodology combines the advantages of protein solubilization, rapid digestion, high peptide coverages, and easy sample preparation for mass spectrometry and liquid chromatography analyses. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
