| Autor: |
Visser NF; Division of Chemistry, Analytical Chemistry and Applied Spectroscopy, Faculty of Sciences, Vrije Universiteit Amsterdam, de Boelelaan 1083, 1081 HV Amsterdam, Netherlands., van Harmelen M, Lingeman H, Irth H |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2003 Oct 15; Vol. 33 (3), pp. 451-62. |
| DOI: |
10.1016/s0731-7085(03)00292-9 |
| Abstrakt: |
An on-line SPE-CE system is described for the determination of insulin derivatives in urine, serum and plasma. By combining techniques based on different separation mechanisms, in this case reversed-phase SPE and CE, a more selective sample clean-up is obtained. The described on-line SPE-CE procedure is able to desalt and clean biological samples, resulting in more repeatable electrophoretic results as well as a good linearity for urine, serum and plasma samples spiked with insulin derivatives, thus proving the elimination of detrimental effects caused by the sample matrix. The on-line SPE-CE system was linear for urine, serum and plasma samples spiked with insulin derivatives between 5 and 80 mg/l. The repeatability in migration time was below 1% relative standard deviation (R.S.D.). The repeatability of the peak was better (<2.4% R.S.D.) when no off-line precipitation reaction (<6.2% R.S.D.) was used, proving the beneficial characteristics of on-line sample pretreatment procedures over off-line sample pretreatment procedures which are prone to sample losses and contamination. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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