| Autor: |
Wardrop J; Scottish Crop Research Institute, Invergowrie, Dundee, DD2 5DA, UK., Fuller J, Powell W, Machray GC |
| Jazyk: |
angličtina |
| Zdroj: |
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2004 Jan; Vol. 108 (2), pp. 343-8. Date of Electronic Publication: 2003 Sep 25. |
| DOI: |
10.1007/s00122-003-1434-6 |
| Abstrakt: |
Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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