| Autor: |
Hibino Y; Cell Biology Division, Faculty of Pharmaceutical Sciences, Toyama Medical & Pharmaceutical University, Japan., Nomura Y, Iwakami N, Sugano N |
| Jazyk: |
angličtina |
| Zdroj: |
Biochemistry international [Biochem Int] 1992 Oct; Vol. 28 (1), pp. 121-7. |
| Abstrakt: |
An Mg(2+)-dependent nuclease was highly purified from rat-liver nuclei. The nuclease activity was enhanced in ultraviolet light (UV)-irradiated dsDNA, but not in unirradiated dsDNA. Irrespective of UV irradiation, ssDNA was readily cleaved by this enzyme. UV-irradiated plasmid DNA was incubated with this enzyme and subjected to the template primer activity assay with a Klenow polymerase. With increasing incubation time, the activity was enhanced in the circular-relaxed and linear forms, but not in the superhelical form. These results implied that this enzyme excises UV-damaged sites in dsDNA to form single strand gaps for repair synthesis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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