| Autor: |
Laxer MA; Department of Infectious and Parasitic Disease Pathology, Armed Forces Institute of Pathology, Washington, DC., D'Nicuola ME, Patel RJ |
| Jazyk: |
angličtina |
| Zdroj: |
The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 1992 Oct; Vol. 47 (4), pp. 450-5. |
| DOI: |
10.4269/ajtmh.1992.47.450 |
| Abstrakt: |
The objective of this project was to demonstrate detection of Cryptosporidium parvum DNA in fixed, paraffin-embedded tissue using the polymerase chain reaction (PCR). DNA was purified from six samples of fixed, paraffin-embedded tissue that were histologically positive for C. parvum and used in the PCR. Previously developed oligonucleotide primers specific for C. parvum were used to amplify a 452-base target sequence, and a 20-base synthetic probe labeled with digoxigenin-11-dUTP was used to detect the amplification product by chemiluminescence. All six samples were positive by PCR; negative controls showed no amplification or hybridization. This approach could provide a sensitive and specific method for detection of parasite material in fixed, paraffin-embedded tissue samples, and prove to be of significant value in retrospective studies of archival material. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
