| Autor: |
Prudnikov IM, Tsyvkin VN, Kastrikina TF |
| Jazyk: |
ruština |
| Zdroj: |
Neirofiziologiia = Neurophysiology [Neirofiziologiia] 1992; Vol. 24 (4), pp. 451-61. |
| Abstrakt: |
Guanosine-5'-triphosphate (GTP)-dependent binding of the agonist D1 dopamine (DA) receptor ligand [H3]-SKF 38393 is described. It is shown that binding of [H3]-SKF 38393 with two different populations takes place in the presence of guanylyl nucleotides, when they are absent--with one population. It is demonstrated using GDP-alpha-P33 binding analysis that the GDP in equilibrium with GTP exchange rate gets higher as a result of activation of DA receptors in the membrane from the mollusc nervous tissues. Influence of the catalytic subunit of the protein kinase A (cPKA) on the [H3]-SKF 38393 and GDP-alpha-P33 is investigated. Obvious influence of the cPKA on [H3]-SKF 38393 binding is not observed, while basal and DA-induced GDP in equilibrium with GTP exchange in membranes of the mollusc nervous tissues is considerably inhibited. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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