Autor: |
Takano E; John Innes Institute, John Innes Centre, Norwich, UK., Gramajo HC, Strauch E, Andres N, White J, Bibb MJ |
Jazyk: |
angličtina |
Zdroj: |
Molecular microbiology [Mol Microbiol] 1992 Oct; Vol. 6 (19), pp. 2797-804. |
DOI: |
10.1111/j.1365-2958.1992.tb01459.x |
Abstrakt: |
Transcription of redD, the activator gene required for production of the red-pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase. The increase in redD expression was followed by transcription of redX, a biosynthetic structural gene, and the appearance of the antibiotic in the mycelium, and coincided with the intracellular appearance of ppGpp. However, ppGpp production elicited either by nutritional shift-down of, or addition of serine hydroxamate to, exponentially growing cultures had no stimulatory effect on redD transcription. The presence of redD on a multicopy plasmid resulted in elevated levels of the redD transcript and production of redX and undecylprodigiosin during exponential growth; the normal growth-phase-dependent production of undecylprodigiosin appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters. |
Databáze: |
MEDLINE |
Externí odkaz: |
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