Localization of the beta A4-crystallin gene (CRYBA4) on human chromosome 22 in the region q11.2-->q13.1.
| Autoři: | van Rens GL; Department of Biochemistry, University of Nijmegen, The Netherlands., Geurts van Kessel AH, Bloemendal H |
|---|---|
| Zdroj: | Cytogenetics and cell genetics [Cytogenet Cell Genet] 1992; Vol. 61 (3), pp. 180-3. |
| Způsob vydávání: | Journal Article; Research Support, Non-U.S. Gov't |
| Jazyk: | English |
| Informace o časopise: | Publisher: Karger Country of Publication: Switzerland NLM ID: 0367735 Publication Model: Print Cited Medium: Print ISSN: 0301-0171 (Print) Linking ISSN: 03010171 NLM ISO Abbreviation: Cytogenet Cell Genet Subsets: MEDLINE |
| Imprint Name(s): | Publication: Basel : Karger Original Publication: Basel, New York, Karger. |
| Výrazy ze slovníku MeSH: | Chromosomes, Human, Pair 22*, Crystallins/*genetics, Blotting, Southern ; DNA Probes/genetics ; Humans ; Hybrid Cells ; Translocation, Genetic/genetics |
| Abstrakt: | The chromosomal localization of the human gene (CRYBA4) coding for the eye lens protein beta A4-crystallin has been carried out using a nearly full-length cDNA clone encoding bovine beta A4-crystallin. A panel of 21 human-mouse or human-hamster hybrid cell lines derived from different parental combinations was characterized with respect to the human chromosomal content and the presence of well established human chromosome-specific markers. These panels were screened for the presence of CRYBA4 using the bovine cDNA clone as a probe. A 100 percent concordance was observed between the presence or absence of the CRYBA4 and human chromosome 22 indicating that the gene resides on this chromosome. By using cell hybrids containing translocated chromosome 22 segments, the localization could be refined to the region 22q11.2-->q13.1. |
| Gene Symbol: | CRYBA4 |
| Substance Nomenclature: | 0 (Crystallins) 0 (DNA Probes) |
| Entry Date(s): | Date Created: 19920101 Date Completed: 19921214 Latest Revision: 20180214 |
| Update Code: | 20240829 |
| DOI: | 10.1159/000133403 |
| PMID: | 1424806 |
| Autor: | van Rens GL; Department of Biochemistry, University of Nijmegen, The Netherlands., Geurts van Kessel AH, Bloemendal H |
| Jazyk: | angličtina |
| Zdroj: | Cytogenetics and cell genetics [Cytogenet Cell Genet] 1992; Vol. 61 (3), pp. 180-3. |
| DOI: | 10.1159/000133403 |
| Abstrakt: | The chromosomal localization of the human gene (CRYBA4) coding for the eye lens protein beta A4-crystallin has been carried out using a nearly full-length cDNA clone encoding bovine beta A4-crystallin. A panel of 21 human-mouse or human-hamster hybrid cell lines derived from different parental combinations was characterized with respect to the human chromosomal content and the presence of well established human chromosome-specific markers. These panels were screened for the presence of CRYBA4 using the bovine cDNA clone as a probe. A 100 percent concordance was observed between the presence or absence of the CRYBA4 and human chromosome 22 indicating that the gene resides on this chromosome. By using cell hybrids containing translocated chromosome 22 segments, the localization could be refined to the region 22q11.2-->q13.1. |
| Databáze: | MEDLINE |
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