| Autor: |
Salter AH; Department of Biochemistry, Arrhenius Laboratory for Natural Sciences, University of Stockholm, Sweden., Newman BJ, Napier JA, Gray JC |
| Jazyk: |
angličtina |
| Zdroj: |
Plant molecular biology [Plant Mol Biol] 1992 Nov; Vol. 20 (3), pp. 569-74. |
| DOI: |
10.1007/BF00040617 |
| Abstrakt: |
cDNA clones encoding the precursor of the Rieske FeS protein of the chloroplast thylakoid membrane have been isolated from a pea leaf cDNA library in lambda gt 11, following screening with antibodies to purified pea chloroplast Rieske FeS protein. The longest cDNA insert of 880 bp encodes a polypeptide of 230 amino acid residues, of which 50 residues constitute an N-terminal cleavable presequence and 180 residues make up the mature protein. Transcription and translation of the cDNA in vitro produced a polypeptide of 26 kDa which was efficiently imported by isolated pea chloroplasts and processed to the mature 20 kDa protein. Southern hybridisation to pea genomic DNA indicated the presence of a single gene encoding the Rieske FeS protein in the haploid genome. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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