| Autor: |
Kirsanova LA, Ablamunits VG, Skaletskiĭ NN, Severin VV |
| Jazyk: |
ruština |
| Zdroj: |
Biulleten' eksperimental'noi biologii i meditsiny [Biull Eksp Biol Med] 1992 Apr; Vol. 113 (4), pp. 404-6. |
| Abstrakt: |
The pancreas from bovine fetuses of 27-35 cm crown-rump length were used as a source of islet cell cultures. Pancreatic tissue was treated by collagenase, filtered through the metal sieve and incubated in MEM for 24 h. Using this method, cultures similar to so-called pseudo-islets were obtained. Aldehyde-fuchsin staining and electron microscopy revealed a significant number of beta-cells within the pseudo-islets, the insulin-producing activity of which was confirmed by RIA. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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