| Autor: |
Prescott CD; Max Planck Institut für Molekulare Genetik, Berlin, Germany., Kornau HC |
| Jazyk: |
angličtina |
| Zdroj: |
Nucleic acids research [Nucleic Acids Res] 1992 Apr 11; Vol. 20 (7), pp. 1567-71. |
| DOI: |
10.1093/nar/20.7.1567 |
| Abstrakt: |
The in vivo expression of mutations constructed within helix 34 of 16S rRNA has been examined together with a nonsense tRNA suppressor for their action at stop codons. The data revealed two novel results: in contrast to previous findings, some of the rRNA mutations affected suppression at UAA and UAG nonsense codons. Secondly, both an increase and a decrease in the efficiency of the suppressor tRNA were induced by the mutations. This is the first report that rRNA mutations decreased the efficiency of a suppressor tRNA. The data are interpreted as there being competition between the two release factors (RF-1 and RF-2) for an overlapping domain and that helix 34 influences this interaction. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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