| Autor: |
Van Lookeren Campagne MM; Department of Anatomy and Cell Biology, Columbia University, New York 10032., Villalba-Díaz F, Meacci E, Manganiello VC, Kessin RH |
| Jazyk: |
angličtina |
| Zdroj: |
Second messengers and phosphoproteins [Second Messengers Phosphoproteins] 1992-1993; Vol. 14 (3), pp. 127-37. |
| Abstrakt: |
A genetic selection procedure has been developed which makes the growth of E. coli dependent on expression of a cGMP phosphodiesterase cDNA. E. coli does not contain a cGMP phosphodiesterase, and guanine auxotrophs cannot extract the guanine from cGMP. If a functional cGMP phosphodiesterase is introduced, then guaA auxotrophs will grow on cGMP as a guanine source. The method also selects GMP synthetase cDNAs, which complement the guanine auxotrophy directly. Expression of a Dictyostelium discoideum or human heart cyclic nucleotide phosphodiesterase cDNA permits growth of the E. coli guaA auxotroph in the presence of cGMP. Several commercial cDNA libraries were corrupt and contained phosphodiesterase and/or GMP synthetase sequences that were from a contaminating DNA source. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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