| Autor: |
Dilworth MJ; School of Biological and Environmental Sciences, Murdoch University, Western Australia., Eldridge ME, Eady RR |
| Jazyk: |
angličtina |
| Zdroj: |
Analytical biochemistry [Anal Biochem] 1992 Nov 15; Vol. 207 (1), pp. 6-10. |
| DOI: |
10.1016/0003-2697(92)90491-o |
| Abstrakt: |
Creatine was identified as a major source of interference with the direct phenol/hypochlorite colorimetric determination of ammonia in nitrogenase reaction mixtures. A method is described for removing other compounds which inhibit color development and for compensating for the interference produced by creatine. This method avoids time-consuming microdiffusion and also routinely makes available the efficiency of ATP hydrolysis coupled to substrate reduction (ATP/2e ratio) with N2 as a reducible substrate. Using this method we determined values for this ratio at 30 degrees C of 4.87 +/- 0.03 during the reduction of protons to H2 and 7.16 +/- 0.14 during the reduction of N2 by the vanadium-containing nitrogenase of Azotobacter chroococcum. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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