| Autor: |
Sorensen KJ; Veterinary Research Laboratory, Harare, Zimbabwe., Madekurozwa RL, Dawe P |
| Jazyk: |
angličtina |
| Zdroj: |
Veterinary microbiology [Vet Microbiol] 1992 Oct; Vol. 32 (3-4), pp. 253-65. |
| DOI: |
10.1016/0378-1135(92)90148-m |
| Abstrakt: |
A blocking ELISA was developed for the detection of antibodies to foot-and-mouth disease virus SAT1, SAT2 and SAT3 and for the quantification of antibodies on a single dilution of serum. The avidin-biotin system was used. The test was compared with the liquid-phase ELISA executed at the World Reference Laboratory for foot-and-mouth disease. It was found to have favourable logistics and combined high specificity with high sensitivity. The quantitative test using a single dilution of serum was resource saving and proved to be a reliable and precise method for the assessment of antibody levels. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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