| Autor: |
Kulakov IuK, Gorelov VN, Motin VL, Brukhanskiĭ GV, Skavronskaia AG |
| Jazyk: |
ruština |
| Zdroj: |
Molekuliarnaia genetika, mikrobiologiia i virusologiia [Mol Gen Mikrobiol Virusol] 1992 Jul-Aug (7-8), pp. 23-7. |
| Abstrakt: |
A non-isotopic amplification system was used to identify and indicate Brucella. The terminal sequences of a protein gene fragment in Brucella outer membrane were identified and direct and reverse primers were chosen for a polymerase chain reaction. (PCR). PCR amplifies a specific DNA fragment, 700 kb in size, only in representatives of the Brucella genus. A probe was design, which is the central part of the amplified DNA fragment, 550 kb in size. Single Brucella cells were detectable with an unlabelled probe in the analyzed samples during hybridization reactions. The system can be recommended for a rapid and reliable analysis in medical and veterinary practice. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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