Use of water-dispersible Fe(2)O(3) nanoparticles with narrow size distributions in isolating avidin.

Autor: Fan J; Department of Chemistry and Biochemistry, Southern Illinois University, Carbondale, IL 62901-4409, USA., Lu J, Xu R, Jiang R, Gao Y
Jazyk: angličtina
Zdroj: Journal of colloid and interface science [J Colloid Interface Sci] 2003 Oct 01; Vol. 266 (1), pp. 215-8.
DOI: 10.1016/s0021-9797(03)00570-8
Abstrakt: Synthetic approaches that vigorously control the microstructures of water-dispersible gamma-Fe(2)O(3) nanoparticles such as size and size uniformity are of importance to the potential biological applications of these nanomaterials. In the present paper, water-dispersible gamma-Fe(2)O(3) nanocrystals with narrow size distributions (bipy-Fe(2)O(3)) were prepared via a site-exchange reaction. These particular materials are superparamagnetic and stable within a wide range of pH. Introduction of the biotin functionality onto the surfaces of bipy-Fe(2)O(3) enabled the affinity isolation of the protein avidin from its incubation solution magnetically with 96% efficiency.
Databáze: MEDLINE