| Autor: |
Zhang Q; Department of Chemistry and Biophysics Research Division, University of Michigan, Ann Arbor, MI 48109, USA., Throolin R, Pitt SW, Serganov A, Al-Hashimi HM |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of the American Chemical Society [J Am Chem Soc] 2003 Sep 03; Vol. 125 (35), pp. 10530-1. |
| DOI: |
10.1021/ja0363056 |
| Abstrakt: |
Approaches developed thus for extracting structural and dynamical information from RDCs have rested on the assumption that motions do not affect molecular alignment. However, it is well established that molecular alignment in ordered media is dependent on conformation, and slowly interconverting conformational substates may exhibit different alignment properties. Neglecting these correlation effects can lead to aberrations in the structural and dynamical analysis of RDCs and diminish the utility of RDCs in probing motions between domains having similar alignment propensities. Here, we introduce a new approach based on measurement of magnetic field induced residual dipolar couplings in nucleic acids which can explicitly take into account such correlations and demonstrate measurements of motions between two "magnetically equivalent" domains in the transactivation response element (TAR) RNA. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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