| Autor: |
Dell' Orbo C; Department of Cytomorphology, University of Cagliari, Italy., Quacci D, Riva A |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of electron microscopy [J Electron Microsc (Tokyo)] 1992 Dec; Vol. 41 (6), pp. 475-9. |
| Abstrakt: |
Alcian blue (AB) was used for scanning electron microscope investigations on metaphyseal cartilage. In the pericellular area three-dimensional network connecting the cell membrane surface to the lacunar wall is evident. The network is formed by very long rod-like filaments about 50 nm thick. The segments may be interpreted as the proteoglycans (PGs) of the pericellular area. In the pericellular area in hypertrophic and degenerative zones, the rod-like segments are closely connected to "chain granules" which are the morphological expression of Ca-P non crystalline compounds. The rod-like segments are not at all evident either in glutaraldehyde-osmium fixed fragments or in predigested-(streptococcal hyaluronidase and chondroitinase) AB stained ones. It is concluded that AB is a good method to detect the three-dimensional spatial disposition of cartilage PGs. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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