The in vitro antitumor activity of vitamins C and K3 against ovarian carcinoma.
| Autor: | von Gruenigen VE; Department of Obstetrics and Gynecology, SUMMA Health System, 525 E. Market Street, Akron, OH 44309, USA. vivian.vongruenigen@uhhs.com, Jamison JM, Gilloteaux J, Lorimer HE, Summers M, Pollard RR, Gwin CA, Summers JL |
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| Jazyk: | angličtina |
| Zdroj: | Anticancer research [Anticancer Res] 2003 Jul-Aug; Vol. 23 (4), pp. 3279-87. |
| Abstrakt: | Background: The objective was to evaluate the cytotoxic effect and mechanism of action of vitamins C (VC) and K3 (VK3) on ovarian carcinoma. Materials and Methods: Cytotoxicity assays were performed on ovarian cancer cell lines with VC, VK3 or a VC/VK3 combination. FIC index was employed to evaluate synergism. Flow cytometry was accomplished at 90% cytotoxic doses. Light, transmission electron microscopy and DNA isolation were performed. Results: Antitumor activity was exhibited by both VC, VK3 and VC/VK3. VC/VK3 demonstrated synergistic activity. VC/VK3 may induce a G1 block in the cell cycle. Combined vitamin treatment resulted in cells that maintain apparently intact nuclei while extruding pieces of organelle-free cytoplasm. Degradation of chromosomal DNA was observed. Conclusion: Cell death (autoschizis) displayed characteristics of both apoptosis and necrosis. The cytotoxic effects observed may enable vitamins C and K3 to play an adjuvant role in the treatment of ovarian cancer. |
| Databáze: | MEDLINE |
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