| Autor: |
Yan K; Institute of Occupational Health and Poisons Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China., Liu B, Xu M, You B, Kang N |
| Jazyk: |
čínština |
| Zdroj: |
Wei sheng yan jiu = Journal of hygiene research [Wei Sheng Yan Jiu] 2003 May; Vol. 32 (3), pp. 180-2. |
| Abstrakt: |
The exogenous gene cyclinD1 cDNA was inserted into eukaryotic expression vector pXJ41-neo by gene transduction and recombination techniques. The recombinant was detected by electrophoresis. Results showed that the recombinant sense plasmid (pXJ41-cyclinD1) was digested by HindIII into 229 bp, 427 bp and 7554 bp; while the recombinant antisense plasmid was digested into 427 bp, 1184 bp and 6599 bp, implying that sense and antisense pXJ41-cyclinD1 eukaryotic expression vectors were successfully recombined. The finding from this study offers an effective tool to reveal the mechanism of the carcinogenesis of environmental and occupational hazards. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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