| Autor: |
Iwata A; The National Institute of Health Sciences, Tokyo, Japan., Satoh K, Murata M, Hikata M, Hayakawa T, Yamaguchi T |
| Jazyk: |
angličtina |
| Zdroj: |
Biological & pharmaceutical bulletin [Biol Pharm Bull] 2003 Aug; Vol. 26 (8), pp. 1065-9. |
| DOI: |
10.1248/bpb.26.1065 |
| Abstrakt: |
To enhance the sensitivity of virus detection by polymerase chain reaction (PCR) and reverse-transcriptional (RT)-PCR, we developed a novel virus-concentration method using sulfonated (SO-) magnetic beads in the presence of divalent cations. In the presence of either Zn(2+) or Cu(2+) ions, we showed that SO-magnetic beads were able to concentrate non-enveloped model viruses, such as porcine parvovirus (PPV) and poliovirus, which were not concentrated by polyethyleneimine (PEI)-magnetic beads.(1)) Using the SO-magnetic beads, the sensitivity of virus genome detection by PCR or RT-PCR can be enhanced. Therefore, an efficient virus concentration method using either SO-magnetic beads or PEI-magnetic beads enhances the sensitivity of virus detection by PCR or RT-PCR. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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