| Autor: |
Mitsui T; Research Center for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8904, Japan., Kimoto M, Harada Y, Sato A, Kitamura A, To T, Hirao I, Yokoyama S |
| Jazyk: |
angličtina |
| Zdroj: |
Nucleic acids research. Supplement (2001) [Nucleic Acids Res Suppl] 2002 (2), pp. 219-20. |
| DOI: |
10.1093/nass/2.1.219 |
| Abstrakt: |
A hydrophobic unnatural nucleoside of 4-propynylpyrrole-2-carbaldehyde (designated as Pa') was synthesized to improve its affinity with a pairing partner, 9-methyl-imidazo[(4,5)-b]pyridine (Q), in enzymatic incorporation. In single-nucleotide insertion experiments using the Klenow fragment, the substrate of Pa' (dPa'TP) was efficiently incorporated opposite Q in the template strand, as compared to the incorporation of pyrrole-2-carbaldehyde (dPaTP), which was previously developed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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