| Autor: |
Sun LV; Department of Genetics and Biostatistics Division, Yale University School of Medicine, New Haven, CT 06520, USA., Chen L, Greil F, Negre N, Li TR, Cavalli G, Zhao H, Van Steensel B, White KP |
| Jazyk: |
angličtina |
| Zdroj: |
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2003 Aug 05; Vol. 100 (16), pp. 9428-33. Date of Electronic Publication: 2003 Jul 22. |
| DOI: |
10.1073/pnas.1533393100 |
| Abstrakt: |
We demonstrate the use of a chromosomal walk (or "tiling path") printed as DNA microarrays for mapping protein-DNA interactions across large regions of contiguous genomic DNA in Drosophila melanogaster. Microarrays were constructed with genomic DNA fragments 430-920 bp in length, covering 2.9 million base pairs of the Adh-cactus region of chromosome 2 and 85,000 base pairs of the 82F region of chromosome 3. We performed DNA localization mapping for the heterochromatin protein HP1 and for the sequence-specific GAGA transcription factor, producing a comprehensive, high-resolution map of in vivo protein-DNA interactions throughout these regions of the Drosophila genome. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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