| Autor: |
Lloyd TL; Glaxo Inc. Research Institute, Research Triangle Park, NC 27709., Perschy TB, Gooding AE, Tomlinson JJ |
| Jazyk: |
angličtina |
| Zdroj: |
Biomedical chromatography : BMC [Biomed Chromatogr] 1992 Nov-Dec; Vol. 6 (6), pp. 311-6. |
| DOI: |
10.1002/bmc.1130060613 |
| Abstrakt: |
A fully automated assay for the analysis of ranitidine in serum and plasma, with and without an internal standard, was validated. It utilizes robotic solid phase extraction with on-line high performance liquid chromatographic (HPLC) analysis. The ruggedness of the assay was demonstrated over a three-year period. A Zymark Py Technology II robotic system was used for serial processing from initial aspiration of samples from original collection containers, to final direct injection onto the on-line HPLC system. Automated serial processing with on-line analysis provided uniform sample history and increased productivity by freeing the chemist to analyse data and perform other tasks. The solid phase extraction efficiency was 94% throughout the assay range of 10-250 ng/mL. The coefficients of variation for within- and between-day quality control samples ranged from 1 to 6% and 1 to 5%, respectively. Mean accuracy for between-day standards and quality control results ranged from 97 to 102% of the respective theoretical concentrations. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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