Monoclonal antibody to fibulin-1 generated by genetic immunization.

Autor: Pupa SM; Molecular Targeting Unit, Department of Experimental Oncology, Istituto Nazionale Tumori, Milan, Italy., Forti S, Invernizzi AM, Giovanazzi R, Twal WO, Argraves WS, Ménard S
Jazyk: angličtina
Zdroj: Journal of cellular biochemistry [J Cell Biochem] 2003 Jul 01; Vol. 89 (4), pp. 647-52.
DOI: 10.1002/jcb.10563
Abstrakt: Fibulin-1 (Fbln-1) is an extracellular matrix (ECM) and plasma glycoprotein. Considering the growing evidence indicating that Fbln-1 plays a role in cancer we sought to develop monospecific antibodies to better facilitate further studies of the function of Fbln-1 in breast cancer. Using a plasmid expression vector encoding full-length human Fbln-1D as an immunogen and CpG oligodeoxyribonucleotides as adjuvant a monoclonal antibody (MAb) against Fbln-1 was produced. This MAb, designated MEM-2 was of IgM isotype and reacted with bacterially expressed Fbln-1. Furthermore, MEM-2 reacted with Fbln-1 expressed in the ECM released by cultured human breast carcinoma SKBR-3 cells in ELISA, and also with Fbln-1 present in SKBR-3 cell extract in immunoprecipitation and Western blotting. MEM-2 also reacted with Fbln-1 in human breast carcinoma specimens. These findings illustrate the utility of genetic immunization as a means of generating monoclonal antibodies to tumor-related ECM proteins. MEM-2 represents a useful new tool for the study of Fbln-1 in breast cancer.
(Copyright 2003 Wiley-Liss, Inc.)
Databáze: MEDLINE
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