| Autor: |
Lapik YR; Laboratory for Molecular Biology, Department of Biological Sciences, University of Illinois at Chicago, Chicago, Illinois 60607, USA., Kaufman LS |
| Jazyk: |
angličtina |
| Zdroj: |
The Plant cell [Plant Cell] 2003 Jul; Vol. 15 (7), pp. 1578-90. |
| DOI: |
10.1105/tpc.011890 |
| Abstrakt: |
Heterotrimeric G proteins are implicated in diverse signaling processes in plants, but the molecular mechanisms of their function are largely unknown. Finding G protein effectors and regulatory proteins can help in understanding the roles of these signal transduction proteins in plants. A yeast two-hybrid screen was performed to search for proteins that interact with Arabidopsis G protein alpha-subunit (GPA1). One of the identified GPA1-interacting proteins is the cupin-domain protein AtPirin1. Pirin is a recently defined protein found because of its ability to interact with a CCAAT box binding transcription factor. The GPA1-AtPirin1 interaction was confirmed in an in vitro binding assay. We characterized two atpirin1 T-DNA insertional mutants and established that they display a set of phenotypes similar to those of gpa1 mutants, including reduced germination levels in the absence of stratification and an abscisic acid-imposed delay in germination and early seedling development. These data indicate that AtPirin1 likely functions immediately downstream of GPA1 in regulating seed germination and early seedling development. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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