| Autor: |
Singer-Sam J; Biology Division, Beckman Research Institute, City of Hope, Duarte, CA 91010., LeBon JM, Dai A, Riggs AD |
| Jazyk: |
angličtina |
| Zdroj: |
PCR methods and applications [PCR Methods Appl] 1992 Feb; Vol. 1 (3), pp. 160-3. |
| DOI: |
10.1101/gr.1.3.160 |
| Abstrakt: |
We have found that the single nucleotide primer extension assay, a PCR-based assay currently used qualitatively to measure allelic differences in DNA, can be used quantitatively to measure allele-specific transcripts differing by only a single nucleotide. We show that total RNA containing the Pgk-1a transcript can be specifically detected even in a 1000-fold excess of RNA containing the Pgk-1b transcript. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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