| Autor: |
Gordeeva OF; Kol'tsov Institute of Developmental Biology, Russian Academy of Sciences, ul. Vavilova 26, Moscow, 119991 Russia. olgagordeeva@yandex.ru, Manuilova ES, Grivennikov IA, Smirnova IuA, Krasnikova NIu, Zinov'eva RD, Khrushchov NG |
| Jazyk: |
ruština |
| Zdroj: |
Ontogenez [Ontogenez] 2003 May-Jun; Vol. 34 (3), pp. 174-82. |
| Abstrakt: |
The expression of regulatory genes of the POU, Pax, Prox, and Ptx gene families was studied at the initial stages of differentiation of murine embryonic stem cells of R1 line. mRNAs were isolated from undifferentiated embryonic stem cells and embryoid bodies formed at the early stages of in vitro differentiation and cDNA sequences were synthesized for comparative PCR analysis of the expression of studied genes. The levels of expression of the gene Oct-4 involved in maintenance of the pluripotent status of embryonic stem cells proved to be practically indistinguishable in undifferentiated cells and embryoid bodies, while the expression of Pax-6 markedly increased in the latter. The levels and patterns of expression of the homeobox transcription factors Prox-1 and Ptx-2 were compared on this cell model for the first time. A probable role of these genes in differentiation of the murine embryonic stem cells is discussed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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