| Autor: |
Ruyani A; Departemen Biologi, Institut Teknologi Bandung Jl, Ganesha No. 10, Labtek XI, Bandung 40132, Indonesia., Sudarwati S, Sutasurya LA, Sumarsono SH, Gloe T |
| Jazyk: |
angličtina |
| Zdroj: |
Toxicological sciences : an official journal of the Society of Toxicology [Toxicol Sci] 2003 Sep; Vol. 75 (1), pp. 148-53. Date of Electronic Publication: 2003 Jun 12. |
| DOI: |
10.1093/toxsci/kfg159 |
| Abstrakt: |
This study is intended to characterize a protein that is linked with mouse limb teratogenicity as the effects of methoxyacetic acid (MAA) treatment. A single dose of MAA (10 mmol/kg body weight) was given by gavage on gestation day (GD) 11, whereas the control group were administered vehicle only. The pregnant mice were killed at 4 h after MAA treatment, and forelimb buds were isolated from both the control and treated group embryos. Proteins from forelimb buds GD 11 + 4 h, which were precipitated out using 40-60% ammonium sulfate, then were analyzed by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (2-D SDS-PAGE) technique. The 2-D gels reveal one protein with 41.6 kDa and pI 6.4, which expression was downregulated after MAA treatment. Tentative protein identification via peptide mass database search and definitive protein identification via a primary sequence database search indicate that the protein matches exactly to 34/67 kDa laminin binding protein (LBP; P14206, SwissProt), which is encoded by p40 gene (MGI:105381). The identity was further verified by Western blotting with an antibody against the 67 kDa LBP. The results suggest that MAA treatment to pregnant mice downregulates the LBP-p40 in the forelimb buds. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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