| Autor: |
Shikova-Lekova E; Institut für Virologie, Medizinische Fakultät, Technische Universität Dresden, Dresden, Germany., Lindemann D, Pietschmann T, Juretzek T, Rudolph W, Herchenröder O, Gelderblom HR, Rethwilm A |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of virology [J Virol] 2003 Jul; Vol. 77 (13), pp. 7677-81. |
| DOI: |
10.1128/jvi.77.13.7677-7681.2003 |
| Abstrakt: |
Replication-competent chimeric retroviruses constructed of members of the two subfamilies of Retroviridae, orthoretroviruses and spumaretroviruses, specifically murine leukemia viruses (MuLV) bearing hybrid MuLV-foamy virus (FV) envelope (env) genes, were characterized. All viruses had the cytoplasmic tail of the MuLV transmembrane protein. In ESL-1, a truncated MuLV leader peptide (LP) was fused to the complete extracellular portion of FV Env, and ESL-2 to -4 contained the complete MuLV-LP followed by N-terminally truncated FV Env decreasing in size. ESL-1 to -4 had an extended host cell range compared to MuLV, induced a cytopathology reminiscent of FVs, and exhibited an ultrastructure that combined the features of the condensed core of MuLV with the prominent surface knobs of FVs. Replication of ESL-2 to -4 resulted in the acquisition of a stop codon at the N terminus of the chimeric Env proteins. This mutation rendered the MuLV-LP nonfunctional and indicated that the truncated FV-LP was sufficient to direct Env synthesis into the secretory pathway. Compared to the parental viruses, the chimeras replicated with only moderate cell-free titers. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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