| Autor: |
Vinogradova KA; M. V. Lomonosov Moscow State University, Moscow., Vlasova II, Sharkova TS, Dodzin ME, Maksimov VN |
| Jazyk: |
ruština |
| Zdroj: |
Antibiotiki i khimioterapiia = Antibiotics and chemoterapy [sic] [Antibiot Khimioter] 2003; Vol. 48 (2), pp. 3-8. |
| Abstrakt: |
Effect of nitrogen sources (organic complexes and mineral salts) on L-glutamate oxidase synthesis by Streptomyces cremeus 510 MGU was studied. Optimal enzyme production was not provided by any single nitrogen source. The most effective combination of nitrogen sources (soy flour, peptone, ammonium sulfate) was elaborated by the mathematical planning method. The results of experiment allowed to enhance biosynthesis of extracellular L-glutamate oxidase to 2.4-2.6 U/mL. It was shown that L-glutamate oxidase of Streptomyces cremeus 510 MGU is highly specific to substance and is stable during storage of filtrated culture with pH 6.8-9.0. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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