| Autor: |
Bilsland JG; Department of Biochemistry and Molecular Biology, Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Harlow, Essex, UK., Harper SJ |
| Jazyk: |
angličtina |
| Zdroj: |
Neuroreport [Neuroreport] 2003 May 23; Vol. 14 (7), pp. 995-9. |
| DOI: |
10.1097/01.wnr.0000070828.57864.ad |
| Abstrakt: |
CEP-1347 inhibits the signalling pathway of c-jun-N-terminal kinase, and is neuroprotective in vivo and in vitro. Embryonic chick dorsal root ganglion neurones are dependent on NGF for survival and neurite outgrowth; NGF withdrawal results in apoptotic cell death. We examined the neuroprotective and neurite outgrowth promoting activity of CEP-1347 in dissociated DRG neurones and in primary DRG explants. CEP-1347 was as effective as NGF in promoting survival of dissociated DRG neurones, but caused only limited neurite outgrowth from DRG explants. When NGF was subsequently added to CEP-1347 treated explants, the outgrowth increased to a similar level to explants which had received NGF throughout. CEP-1347 may be a useful tool to maintain viable DRG explants to allow evaluation of neurite outgrowth promoting compounds and dissection of survival and neurite outgrowth signalling pathways. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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