Elution of metronidazole and gentamicin from polymethylmethacrylate beads.

Autor: Ramos JR; Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, VA 24061, USA., Howard RD, Pleasant RS, Moll HD, Blodgett DJ, Magnin G, Inzana TJ
Jazyk: angličtina
Zdroj: Veterinary surgery : VS [Vet Surg] 2003 May-Jun; Vol. 32 (3), pp. 251-61.
DOI: 10.1053/jvet.2003.50024
Abstrakt: Objective: To characterize the elution and bioactivity of metronidazole and gentamicin sulfate polymerized, individually and in combination, with polymethylmethacrylate (PMMA).
Study Design: In vitro experimental study.
Methods: PMMA beads containing metronidazole (3 concentrations), gentamicin sulfate, or metronidazole and gentamicin sulfate were immersed in 5 mL of phosphate-buffered saline in triplicate. Eluent was replaced at specified time intervals for 1 or 21 days, and antibiotic concentrations were measured by high-performance liquid chromatography. Changes in antibiotic bioactivity attributable to polymerization or copolymerization of the antibiotics with PMMA, ethylene oxide sterilization, and storage of AIPMMA beads containing metronidazole were evaluated.
Results: Antibiotic elution patterns were similar for all groups. Day 1 elution for groups containing metronidazole or gentamicin individually represented a mean 63%-66% and 79%, respectively, of the 21-day total. Approximately 50% of the day 1 elution occurred during the first hour. The elution of metronidazole was dose dependent. The elution of metronidazole (day 3-21) and gentamicin (all days) was significantly greater when metronidazole and gentamicin were combined (P <.05). The addition of metronidazole delayed polymerization of PMMA. Neither polymerization nor copolymerization of metronidazole and gentamicin with PMMA, gas sterilization, or 2-month storage of beads containing metronidazole significantly affected antimicrobial bioactivity.
Conclusions: Metronidazole elution from PMMA was dose dependent. Copolymerization of metronidazole and gentamicin sulfate in PMMA resulted in increased rates of elution. Intraoperative preparation of metronidazole-impregnated PMMA beads is not practical, but sterilization and storage for 2 months should not affect efficacy.
Clinical Relevance: The local delivery of biologically active metronidazole and gentamicin by elution from PMMA is feasible.
(Copyright 2003 by The American College of Veterinary Surgeons)
Databáze: MEDLINE
Externí odkaz: