| Autor: |
Zheng Y; Department of Ion Channels, Merck Research Laboratories, Rahway, NJ 07065, USA., Priest B, Cully DF, Ludmerer SW |
| Jazyk: |
angličtina |
| Zdroj: |
Insect biochemistry and molecular biology [Insect Biochem Mol Biol] 2003 Jun; Vol. 33 (6), pp. 595-9. |
| DOI: |
10.1016/s0965-1748(03)00038-9 |
| Abstrakt: |
The American dog tick Dermacentor variabilis is a major transmitter of bacterial and viral pathogens in human and animal populations, and compounds active against this species would benefit both human and animal health. Invertebrate GABA-gated chloride channels are validated targets of commonly used insecticides and acaricides. We cloned a novel member of the invertebrate GABA-gated chloride channel gene family from Dermacentor variabilis, RdlDv. The closest homologue of the predicted gene product of RdlDv is the RDL protein encoded by the GABA-gated chloride channel gene Drosophila Rdl (Resistance to Dieldrin), with which it shares 64% amino acid identity. When expressed in Xenopus oocytes, RdlDv produces GABA-activated currents blocked by the known insecticides and RDL antagonists fipronil and picrotoxinin. These results suggest that RdlDv encodes a GABA-gated chloride channel subunit, making it a potential target for compounds active against the tick D. variabilis. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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