| Autor: |
Rigos G; Laboratory of Fish Nutrition and Pathology, National Centre for Marine Research, Aghios Kosmas 16604, Ellinikon, Attiki, Greece. grigos@ncmr.gr, Tyrpenou AE, Nengas I, Yiagnisis M, Koutsodimou M, Alexis M, Troisi GM |
| Jazyk: |
angličtina |
| Zdroj: |
Diseases of aquatic organisms [Dis Aquat Organ] 2003 Mar 17; Vol. 54 (1), pp. 35-41. |
| DOI: |
10.3354/dao054035 |
| Abstrakt: |
The present study investigated the kinetic profile of flumequine (FLU) in gilthead sea bream Sparus aurata (170 g) held at 19 degrees C and evaluated its in vitro efficacy against important bacterial diseases in Mediterranean mariculture. Following a single intravascular injection (10 mg kg(-1) fish), the distribution half-life (t1/2alpha) and the half-life of the terminal phase of elimination (t1/2gamma) of the drug were 0.2 and 30 h respectively. Tissue penetration of FLU was low, since both the apparent distribution volume of the drug at steady-state (Vd(SS)) and the apparent volume of the central compartment (Vc) were small (0.57 and 0.15 l kg(-1)). The mean residence time (MRT) was short (11 h) and the total clearance (CL(T)) of the drug was slow (0.05 l kg(-1) h(-1)). Following oral administration (20 mg kg(-1)), the bioavailability (F %) of FLU was 29% and the maximum plasma concentration was 1.7 microg ml(-1). The minimum inhibitory concentration (MIC) of the drug in distilled water supplemented with 2% NaCl against Vibrio anguillarum Serotype 1b, Photobacterium damsela ssp. piscicida, V. alginolyticus, V. damsela and V. fluvialis was 0.15, 0.3, 1.2, 0.019 and 0.15 microg ml(-1) respectively. The addition however of 10 mM Ca2+ and 55 mM Mg2+ to the medium resulted in an 8- to >120-fold reduction in FLU activity. The results indicate that FLU has an adequate kinetic profile in gilthead sea bream and that marine cations induce a significant impact on the activity of FLU, rendering its use against bacterial pathogens questionable. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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