| Autor: |
Grimm EA, Silver HK, Roth JA, Chee DO, Gupta RK, Morton DL |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of cancer [Int J Cancer] 1976 May 15; Vol. 17 (5), pp. 559-64. |
| DOI: |
10.1002/ijc.2910170503 |
| Abstrakt: |
Spent tissue culture medium (CDM-S) removed from a single cell line of human malignant melanoma grown in serum-free CDM, contained tumor-associated antigenic activity. Antibodies to CDM-S measured by complement fixation were detected in 44% (31/70) melanoma, 55% (15/27) sarcoma, 63% (24/38) carcinoma and 15% (11/72) normal sera. Delayed cutaneous hypersensitivity reactions (DCHR) were demonstrated in 4/5 melanoma patients at a 500 mug dose, 3/5 at a 100 mug dose and in 1/7 carcinoma patients at the 500 mug dose. One ml of CDM-S was shown to contain antigen equivalent to that obtained from the membranes of 2.9 X 10(7) tissue-cultured melanoma cells. After purification, 84% (16/19) sera from melanoma patients, 66% (12/18) from sarcoma and carcinoma patients and 8% (2/26) from normal controls were positive to the antigen by complement fixation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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