| Autor: |
Colding H; Institute of Medical Microbiology and Immunology, Panum Institute, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. H.Colding@immi.ku.dk, Hartzen SH, Mohammadi M, Herup K, Kristiansen JE, Andersen LP |
| Jazyk: |
angličtina |
| Zdroj: |
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases [Clin Microbiol Infect] 2003 Jan; Vol. 9 (1), pp. 57-60. |
| DOI: |
10.1046/j.1469-0691.2003.00476.x |
| Abstrakt: |
Recently, PCR-restriction fragment length polymorphism (PCR-RFLP) of the urease genes of Helicobacter pylori was evaluated in a meta-analysis; acceptable discriminatory indices of the ureAB and C genes were found. In the present investigation, we found a discriminatory index of 0.95 for 191 unrelated clinical H. pylori isolates with PCR-RFLP typing of the ureB gene (933 bp), combining the results obtained with restriction enzymes HaeIII and Sau3A, and a mixture of the enzymes. We therefore find that PCR-RFLP typing of the ureB gene of H. pylori with restriction enzymes HaeIII and Sau3A is comparable to typing of other H. pylori urease genes. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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