| Autor: |
Arnold S; Microparticle Photophysics Lab MP3L), Polytechnic University, Brooklyn, New York 11201, USA. arnold@photon.poly.edu, Khoshsima M, Teraoka I, Holler S, Vollmer F |
| Jazyk: |
angličtina |
| Zdroj: |
Optics letters [Opt Lett] 2003 Feb 15; Vol. 28 (4), pp. 272-4. |
| DOI: |
10.1364/ol.28.000272 |
| Abstrakt: |
Biosensors based on the shift of whispering-gallery modes in microspheres accompanying protein adsorption are described by use of a perturbation theory. For random spatial adsorption, theory predicts that the shift should be inversely proportional to microsphere radius R and proportional to protein surface density and excess polarizability. Measurements are found to be consistent with the theory, and the correspondence enables the average surface area occupied by a single protein to be estimated. These results are consistent with crystallographic data for bovine serum albumin. The theoretical shift for adsorption of a single protein is found to be extremely sensitive to the target region, with adsorption in the most sensitive region varying as 1/R(5/2). Specific parameters for single protein or virus particle detection are predicted. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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