An improved method for examination of cerebrospinal fluid cells.

Autor: Stokes HB, O'Hara CM, Buchanan RD, Olson WH
Jazyk: angličtina
Zdroj: Neurology [Neurology] 1975 Oct; Vol. 25 (10), pp. 901-6.
DOI: 10.1212/wnl.25.10.901
Abstrakt: A new collection technique allows cytologic examination up to 2 weeks after lumbar puncture without loss of cells or morphologic detail. Cerebrospinal fluid (CSF) was allowed to flow directly from a lumbar puncture needle into a solution of Carbowax in ethanol and was then processed with a cytocentrifuge. Two groups of patients were studied -- those undergoing spinal anesthesia and those having myelography for low back pain. Lymphocytes, polymorphonuclear neutrophils, and ependymal cells were regularly found in the CSF; a definite difference was found in the quantity and cell types in the two patient populations. The proposed method may be sensitive enough to aid in the identification of other neurologic diseases with low cell counts.
Databáze: MEDLINE