ADAM7 (a disintegrin and metalloprotease 7) mRNA is suppressed in mouse epididymis by neonatal exposure to Diethylstilbestrol.

Autor: Adachi T; Department of Bioenvironmental Medicine, Graduate School of Medicine, Chiba University, Chiba 260-8531, Japan., Matsuno Y, Sugimura A, Takano K, Koh KB, Sakurai K, Shibayama T, Iguchi T, Mori C, Komiyama M
Jazyk: angličtina
Zdroj: Molecular reproduction and development [Mol Reprod Dev] 2003 Apr; Vol. 64 (4), pp. 414-21.
DOI: 10.1002/mrd.10235
Abstrakt: We investigated whether neonatal exposure to diethylstilbestrol (DES) induces the alteration of mRNA expression in adult mouse epididymis, which plays an important role in sperm maturation. Using a cDNA subtraction method, we isolated 15 changed gene clones in neonatally DES-treated mouse epididymides, and we found a clone homologous with a disintegrin and metalloprotease (ADAM) 7 in the epididymis, as a suppressed gene, by means of neonatal DES treatment in 8-week-old mice. Indeed, it was confirmed by Northern blot analysis that the ADAM7 mRNA expression in the epididymis was at a lower level in neonatally DES-treated mice than in non-treated mice. Moreover, in situ hybridization analysis and real-time reverse transcription and polymerase chain reaction (real-time RT-PCR) revealed that ADAM7 expression was markedly reduced in the corpus region of the epididymis of DES-treated mice as compared with non-treated mice. Our results suggest that neonatal exposure to DES leads to the suppression of ADAM7 expression in the epididymis in the long term. ADAM7 gene expression might be a biological marker of fetal or neonatal exposure to estrogenic compounds, including endocrine disruptors.
(Copyright 2003 Wiley-Liss, Inc.)
Databáze: MEDLINE
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