| Autor: |
Iyer JL; Food and Fermentation Technology Division, Institute of Chemical Technology, University of Mumbai, Nathalal Parikh Marg, Matunga, Mumbai 400 019, Maharashtra, India., Shetty P, Pai JS |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of industrial microbiology & biotechnology [J Ind Microbiol Biotechnol] 2003 Jan; Vol. 30 (1), pp. 47-51. Date of Electronic Publication: 2003 Jan 03. |
| DOI: |
10.1007/s10295-002-0009-x |
| Abstrakt: |
Cyclodextrin glucanotransferase (CGTase) from Bacillus circulans (ATCC 21783) was immobilised on a silica-based support: purified seasand. Although adsorption of 98% was achieved, considerable desorption was encountered. This problem was minimised by crosslinking the adsorbed enzyme with glutaraldehyde. The immobilised enzyme after crosslinking could be used repeatedly for cyclodextrin (CD) production in a batch process. The activity retention was 80% at the end of the eighth cycle. The immobilised enzyme showed a shift in the pH optimum towards the alkaline side and also an improvement in the pH stability compared to the free enzyme. It catalysed the formation of beta-CD as a major product. A significant amount of alpha-CD production was also observed on prolonged incubation. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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