| Autor: |
Kumei Y; Graduate School of Tokyo Medical and Dental University, Tokyo 113-8549, Japan. kumei.bch@tmd.ac.jp, Nakamura H, Morita S, Akiyama H, Hirano M, Ohya K, Shinomiya K, Shimokawa H |
| Jazyk: |
angličtina |
| Zdroj: |
Annals of the New York Academy of Sciences [Ann N Y Acad Sci] 2002 Nov; Vol. 973, pp. 75-8. |
| DOI: |
10.1111/j.1749-6632.2002.tb04609.x |
| Abstrakt: |
The primary culture of rat osteoblasts was treated with 1alpha,25 dihydroxyvitamin D(3) and fixed with guanidine isothiocyanate solution during a space shuttle flight. The mRNA levels were analyzed by quantitative reverse transcription-polymerase chain reaction. Microgravity decreased the mRNA levels of insulin-like growth factor-I (IGF-I) and increased reciprocally the IGF-I receptor mRNA levels, as compared to the ground (1 x g) control. Microgravity completely suppressed the mRNA expression of the insulin receptor substrate-1, the postreceptor signaling molecule of IGF-I. Microgravity might deteriorate the action and signaling of IGF-I in rat osteoblasts. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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