| Autor: |
Bacanamwo M; Department of Biochemistry and Interdisciplinary Plant Group, 117 Schweitzer Hall, University of Missouri-Columbia, Columbia, MO 65211, USA., Witte CP, Lubbers MW, Polacco JC |
| Jazyk: |
angličtina |
| Zdroj: |
Molecular genetics and genomics : MGG [Mol Genet Genomics] 2002 Dec; Vol. 268 (4), pp. 525-34. Date of Electronic Publication: 2002 Nov 12. |
| DOI: |
10.1007/s00438-002-0769-z |
| Abstrakt: |
Plant orthologs of the bacterial urease accessory genes ureD and ureF, which are required for the insertion of the nickel ion at the active site, have been isolated from soybean ( Glycine max L. Merr.), tomato ( Lycopersicon esculentum) and Arabidopsis thaliana. The functionality of soybean UreD and UreF was tested by measuring their ability to complement urease-negative mutants of Schizosaccharomyces pombe, a eukaryote which produces a "plant-like" urease of ~90 kDa. The S. pombe ure4 mutant was complemented by a 12-kb fragment of S. pombe genomic DNA, which was shown by PCR to contain a putative ureD gene. However, ure4 was not complemented by a UreD cDNA soybean, expressed under the control of a strong promoter. In contrast, an S. pombe ure3 mutation was complemented by both a 10-kb fragment of S. pombe DNA containing ureF and the UreF cDNA from soybean. Soybean Eu2 is a candidate urease accessory gene; its product cooperates with the Eu3 protein in activating apourease in vitro. However, the sequences of UreD and UreF transcripts from two eu2/eu2 mutants, recovered as RT-PCR products, revealed no mutational alteration, suggesting that Eu2 encodes neither UreD nor UreF. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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