| Autor: |
Haig DM; The Moredun Research Institute, International Research Center, Pentlands Science Park, Bush Loan, EH26 0PZ, Penicuik, UK.haigd@mri.sari.ac.uk, Thomson J, McInnes CJ, Deane DL, Anderson IE, McCaughan CA, Imlach W, Mercer AA, Howard CJ, Fleming SB |
| Jazyk: |
angličtina |
| Zdroj: |
Virus research [Virus Res] 2002 Dec; Vol. 90 (1-2), pp. 303-16. |
| DOI: |
10.1016/s0168-1702(02)00252-6 |
| Abstrakt: |
Orf virus causes pustular skin lesions (orf) in sheep, goats and humans. The virus encodes an interleukin-10 (orfvIL-10) that is identical in amino acid composition to ovine IL-10 (ovIL-10) over the C terminal two-thirds of the polypeptide, but not in the N terminal third. The immuno-suppressive and immuno-stimulatory activities of orfvIL-10 and ovIL-10 were compared. Both orfvIL-10 and ovIL-10 inhibited TNF-alpha and IL-8 cytokine production from stimulated ovine macrophages and keratinocytes and IFN-gamma and GM-CSF production from peripheral blood lymphocytes. OrfvIL-10 and ovIL-10 co-stimulated both ovine and murine mast cell proliferation in conjunction with IL-3 (ovine) or IL-4 (murine). Isoleucine at position 87 (Ile(87)) of the mature human IL-10 (huIL-10) has been reported as essential for the immuno-stimulatory activity of huIL-10. In spite of the differences in amino acids within the N-terminal third of orfvIL-10 compared with ovIL-10 and substitution of Ile(87) with Ala(87) in ovIL-10, these variants of ovIL-10 and orfvIL-10 all co-stimulated mast cell proliferation and inhibited macrophage IL-8 production. As ovIL-10 and orfvIL-10 have a similar structure to huIL-10 and conserved receptor-binding residues, it was concluded that Ile(87) is not essential for IL-10 immuno-stimulatory activity. Finally, ovine keratinocytes do not express ovIL-10. This might explain why orf virus has evolved a viral IL-10. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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Full Text from ScienceDirect