| Autor: |
Larina ON, Sidorenko LA, Sukhachev VI, Manuilova ES, Inozemtseva LS, Grivennikov IA |
| Jazyk: |
ruština |
| Zdroj: |
Aviakosmicheskaia i ekologicheskaia meditsina = Aerospace and environmental medicine [Aviakosm Ekolog Med] 2002; Vol. 36 (4), pp. 54-8. |
| Abstrakt: |
Unlike the monolayer cultures, three-dimensional (multilayer) cultures of living cells make it possible to more closely imitate events occurring in native tissues. Rotating cultivators for three-dimensional cultures create analogous conditions of reduced gravity and, therefore, can be used in ground-based investigations of the biological effects of space flight. We have designed a disposable cultivation cell with diffused gas exchange and a device to rotate the cell at a controlled speed about the horizontal axis. Immortalized CH-fibroblasts were attached to a glass carrier pretreated with polylysine. Cultivation was performed for 2-3 days at 8 rot./min. A rapid growth of three-dimensional cell complexes was observed during the cultivation. The investigation demonstrated that multilayer cultures of immortalized CH-fibroblasts represent a convenient model for studies in cell biology and development of technologies of growing d-dimensional living cell complexes as a source of implants from autologic cells (including stem cells) thus resolving the problem of donator materials. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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