| Autor: |
Frederickson CJ; Center for Biomedical Engineering, and Department of Anatomy and Neuroscience, University of Texas Medical Branch, 625 Jennie Sealy Hospital, Galveston 77555, USA. chris@neurobiotex.com, Cuajungco MP, LaBuda CJ, Suh SW |
| Jazyk: |
angličtina |
| Zdroj: |
Neuroscience [Neuroscience] 2002; Vol. 115 (2), pp. 471-4. |
| DOI: |
10.1016/s0306-4522(02)00399-8 |
| Abstrakt: |
One of us showed previously [Cuajungco and Lees (1998) Brain Res. 799, 188-129] that nitric oxide injected into the cerebrum in vivo causes zinc staining to appear in the somata of neurons and suggested that this staining of somata might be accompanied by a depletion (release) of zinc from axon terminals. In the present study, we confirm earlier results and report that there is a dramatic loss (apparent release) of histologically reactive zinc from the boutons of zinc-containing axons induced by infusion of nitric oxide into the brain in vivo. Rats were anesthetized with halothane and a cannula was inserted into the hippocampus. Either nitric oxide donor (spermineNONOate, 100 mM/2 microl) or control (spermine, 100 mM/2 l) was infused into the hippocampus or the cerebellar cortex. Two hours after infusion, N-(6-methoxy-8-quinolyl)-para-toluenesulfonamide (TSQ) staining for zinc in the brains revealed that sperminenitric oxide, but not control (spermine only) produced up to 95% depletion of zinc staining from the zinc-containing boutons. TSQ-positive neurons were also conspicuous throughout injection sites, in both the cerebral cortex and in the cerebellar cortex, where the Purkinje neurons were especially vivid, despite the scarcity of zinc-containing axonal boutons. It is suggested that the TSQ-stainable zinc in somata might represent intracellular stores mobilized from within or permeating extracellular stores. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
|
Full Text from ScienceDirect