Measurement of glial fibrillary acidic protein in blood: an analytical method.

Autor: van Geel WJ; Laboratory of Pediatrics and Neurology, University Medical Centre Nijmegen, Reinier Postlaan 4, 6525 GC Nijmegen, The Netherlands. w.vangeel@cukz.umcn.nl, de Reus HP, Nijzing H, Verbeek MM, Vos PE, Lamers KJ
Jazyk: angličtina
Zdroj: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2002 Dec; Vol. 326 (1-2), pp. 151-4.
DOI: 10.1016/s0009-8981(02)00330-3
Abstrakt: Background: In the present study, a new assay for the measurement of glial fibrillary acidic protein (GFAP) in human blood is described. The aim of the study was to present the characteristics of a new GFAP assay for blood analysis.
Methods: The method, a newly developed enzyme-linked immunosorbent assay (ELISA), was validated using blood samples from healthy blood donors and from patients with severe traumatic brain injury (TBI).
Results: 22 out of 72 healthy blood donors had a detectable GFAP level. The reference values (percentiles) are as follows: P50=0.15 microg/l and P95=0.49 microg/l; range 0.15-0.76 microg/l.
Conclusions: GFAP in blood might be a promising new marker for astrocyte involvement after acute brain damage.
Databáze: MEDLINE
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