| Autor: |
Chekanov AV; Research Institute of Physical and Chemical Medicine, Malaya Pirogovskaya ul. 1 a, Moscow, 119992 Russia., Panasenko OM, Osipov AN, Arnhold J, Kazarinov KD, Vladimirov IuA, Sergienko VI |
| Jazyk: |
ruština |
| Zdroj: |
Biofizika [Biofizika] 2002 Sep-Oct; Vol. 47 (5), pp. 787-94. |
| Abstrakt: |
The interaction of hypochlorite (HOCl/OCl-) with tert-butyl hydroperoxide ((CH3)3COOH) was investigated by chemiluminescence. It was shown that the addition of HOCl/OCl- to (CH3)3COOH induces a fast chemiluminescent flash. The intensity of this flash increases with the increase in both HOCl/OCl- and (CH3)3COOH concentration. The chemiluminescence is quenched in a concentration-dependent manner in the presence of free radical spin traps N-tert-butyl nitrone and alpha-(4-pyridyl-1-oxyl)-N-tert-butyl nitrone. This fact proves that free radicals take part in the interaction of HOCl/OCl- and (CH3)3COOH. Hypochlorite yielded a very similar chemiluminescence spectrum in its reaction with (CH3)3COOH as Ce4+. It differed considerably from the spectrum in the system H2O2 and HOCl/OCl-. It is well known that the interaction of Ce4+ and (CH3)3COOH produces peroxyl radicals. These results confirm the hyothesis that the interaction of HOCl/OCl- and (CH3)3COOH is mediated by peroxyl radicals. Thus, organic hydroperoxides always present in unsaturated lipids can induce lipid peroxidation processes in the reaction with HOCl/OCl-. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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